pUbiSXR
(Plasmid #64403)

• Purpose: Ubiquitin promoter driving synthetic xylanase (sXynA) gene. Can be used as a reporter of promoter activity in plants by replacing Ubi promoter with sequence of interest.
• Depositing Lab: Claudia Vickers
• Publication: Vickers et al Plant Cell Rep. 2003 Sep;22(2):135-40. Epub 2003 Jul 4.

Backbone

• Vector backbone: pSP72
• Vector type: Plant Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: xylanase
• Alt name: sXynA
• Species: Synthetic; Neocallimastix patriciarum
• Mutation: codon optimized for plant expression
• Promoter: Plant ubiquitin (Ubi-1) promoter (with untranslated region intron)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Supplemental Documents:
  • pUbiSXR genbank file

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plant ubiquitin (Ubi-1) promoter (with untranslated region intron) driving expression of a synthetic, codon-optimised xylanase gene followed by an rbcS terminator. The xylanase is used as a reporter gene for quantitative promoter expression in plant tissues.