pUbiGUSPlus
(Plasmid #64402)

• Purpose: Ubiquitin promoter driving synthetic optimised GUSPlus gene. Can be used as a reporter of promoter activity in plants by replacing Ubi promoter with sequence of interest.
• Depositing Lab: Claudia Vickers
• Publication: Vickers et al Plant Cell Rep. 2003 Sep;22(2):135-40. Epub 2003 Jul 4.

Backbone

• Vector backbone: pSP72
• Vector type: Plant Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ubiquitin (Ubi-1) promoter
• Species: maize
• Promoter: Ubi-1
• Tag / Fusion Protein:
  • GUSPlus reporter

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: M13-F20
• 3′ sequencing primer: M13R

Resource Information

• Supplemental Documents:
  • pUbiGUSPlus genbank file

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plant ubiquitin (Ubi-1) promoter (with untranslated region intron) driving expression of a synthetic, optimised GUSPlus gene (CAMBIA) followed by an NOS terminator. The GUSPlus gene is used as a reporter gene for quantitative promoter expression in plant tissues.