pGFPGUSPlus
(Plasmid #64401)

• Purpose: binary plant vector expressing GFP and GUS, both driven by a 35S promoter
• Depositing Lab: Claudia Vickers
• Publication: Vickers et al Biotechnol Lett. 2007 Nov;29(11):1793-6. Epub 2007 Aug 9.

Backbone

• Vector backbone: pCAMBIA1305.1
• Backbone manufacturer: CAMBIA
• Vector type: Plant Expression ; binary vector for plant transformation
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: GFP
• Mutation: S65T
• GenBank ID: ABQ22954.1
• Promoter: 35S

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: M13-F20
• 3′ sequencing primer: M13-R

Gene/Insert 2

• Gene/Insert name: GUSPlus
• GenBank ID: ABQ22951.1
• Promoter: 35S
• Tag / Fusion Protein:
  • His (C terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: NA

Resource Information

• Supplemental Documents:
  • pGFPGUSplus genbank file
• A portion of this plasmid was derived from a plasmid made by: CAMBIA
• Articles Citing this Plasmid:
  • 8 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

GenBank record number for the full plasmid seqeunce: EF546437.1

The 35S:GFP:NOS expression cassette from p35SsGFP was cloned into pCAMBIA1305.1 upstream and in the opposite orientation to the 35S:GUSPlus:NOS cassette. Expression of both reporter genes benefits from the enhancer elements found in the two 35S promoters.