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Addgene

pOGGUSPlus
(Plasmid #64400)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 64400 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGGFPR (pSP72)
  • Modifications to backbone
    GFP replaced with e GUSPlus coding region and the nopaline synthase (NOS) terminator region isolated from pCAMBIA 1305.1 (CAMBIA, www.cambia.org).
  • Vector type
    Plant Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Oat globulin Glo1 promoter
  • Alt name
    Glo1
  • Species
    Avena sativa
  • Insert Size (bp)
    960
  • GenBank ID
    AY795082.1
  • Promoter Oat globulin Glo1
  • Tag / Fusion Protein
    • GUSplus (modified Staphylococcus sp. b-glucuronidase) (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOGGUSPlus was a gift from Claudia Vickers (Addgene plasmid # 64400 ; http://n2t.net/addgene:64400 ; RRID:Addgene_64400)

  • For your References section:

    A novel cis-acting element, ESP, contributes to high-level endosperm-specific expression in an oat globulin promoter. Vickers CE, Xue G, Gresshoff PM. Plant Mol Biol. 2006 Sep;62(1-2):195-214. Epub 2006 Aug 17. 10.1007/s11103-006-9014-1 PubMed 16915522
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