pHMWGFPR
(Plasmid
#64396)
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PurposeHigh molecular weight glutenin gene promoter driving GFP expression
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 64396 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGGFPR (pSP72)
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Vector typePlant Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHigh molecular weight glutenin gene promoter
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SpeciesTriticum aestivum
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Insert Size (bp)1358
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GenBank IDAY795083.1
- Promoter High molecular weight glutenin gene promoter
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Tag
/ Fusion Protein
- GFP (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer EGFP-C (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
High molecular weight glutenin gene promoter from wheat was fused to green fluorescent protein, followed by an rbcS terminator
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHMWGFPR was a gift from Claudia Vickers (Addgene plasmid # 64396 ; http://n2t.net/addgene:64396 ; RRID:Addgene_64396) -
For your References section:
A novel cis-acting element, ESP, contributes to high-level endosperm-specific expression in an oat globulin promoter. Vickers CE, Xue G, Gresshoff PM. Plant Mol Biol. 2006 Sep;62(1-2):195-214. Epub 2006 Aug 17. 10.1007/s11103-006-9014-1 PubMed 16915522