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PurposeFlag-tagged biotinylation enzyme for co-expression with bio-proteins
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 64395 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepTT3
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Backbone manufacturerYves Durocher
- Backbone size w/o insert (bp) 6000
- Total vector size (bp) 7043
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameBirA
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Alt nameBiotin ligase
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SpeciesE. coli
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Insert Size (bp)1000
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Entrez GenebirA (a.k.a. b3973, ECK3965, bioR, dhbB)
- Promoter CMV
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Tag
/ Fusion Protein
- Flag tag (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Sal1 (destroyed during cloning)
- 3′ cloning site Xba1 (not destroyed)
- 5′ sequencing primer CMV forward (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
secreted BirA-Flag was a gift from Gavin Wright (Addgene plasmid # 64395 ; http://n2t.net/addgene:64395 ; RRID:Addgene_64395) -
For your References section:
Large-scale screening for novel low-affinity extracellular protein interactions. Bushell KM, Sollner C, Schuster-Boeckler B, Bateman A, Wright GJ. Genome Res. 2008 Apr;18(4):622-30. Epub 2008 Feb 22. 10.1101/gr.7187808 PubMed 18296487
Map uploaded by the depositor.
