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Addgene

pCYC1m_yeGFP
(Plasmid #64389)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 64389 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS406
  • Backbone size w/o insert (bp) 4500
  • Total vector size (bp) 5500
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    yeast enhanced GFP
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1000
  • Promoter pCYC1

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CTTTTCTCTAAATATTCTTTCCTTATACATTAGG
  • 3′ sequencing primer GGGACCTAGACTTCAGGTTGTCTAACTCC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCYC1m_yeGFP was a gift from Timothy Lu (Addgene plasmid # 64389 ; http://n2t.net/addgene:64389 ; RRID:Addgene_64389)

  • For your References section:

    Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas. Farzadfard F, Perli SD, Lu TK. ACS Synth Biol. 2013 Sep 11. 10.1021/sb400081r PubMed 23977949
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