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8798 GFP-KrasG12C_2B_hygro
(Plasmid #64376)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 64376 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    8200 Bicistronic_GFP_ires_hygro
  • Backbone manufacturer
    Theodora Agalioti
  • Backbone size w/o insert (bp) 8200
  • Total vector size (bp) 8798
  • Modifications to backbone
    AgeI, HpaI
  • Vector type
    Retroviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mouse Kras2B
  • Alt name
    Kras
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1356
  • Mutation
    changed glycine 12 to cysteine
  • Entrez Gene
    Kras (a.k.a. K-Ras, K-Ras 2, K-ras, Ki-ras, Kras-2, Kras2, c-K-ras, c-Ki-ras, p21B, ras)
  • Tag / Fusion Protein
    • GFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site SmaI (destroyed during cloning)
  • 5′ sequencing primer ccctcactccttctctagg
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This construct was created in G. Stahopoulos lab in Patras, by Theodora Agalioti and represents a retroviral expression vector of GFP-Kras2B G12C mutant oncogene in a bicistronic fashion along with hygromycin resistance gene

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    8798 GFP-KrasG12C_2B_hygro was a gift from Georgios Stathopoulos (Addgene plasmid # 64376 ; http://n2t.net/addgene:64376 ; RRID:Addgene_64376)

  • For your References section:

    Mutant KRAS promotes malignant pleural effusion formation. Agalioti T, Giannou AD, Krontira AC, Kanellakis NI, Kati D, Vreka M, Pepe M, Spella M, Lilis I, Zazara DE, Nikolouli E, Spiropoulou N, Papadakis A, Papadia K, Voulgaridis A, Harokopos V, Stamou P, Meiners S, Eickelberg O, Snyder LA, Antimisiaris SG, Kardamakis D, Psallidas I, Marazioti A, Stathopoulos GT. Nat Commun. 2017 May 16;8:15205. doi: 10.1038/ncomms15205. 10.1038/ncomms15205 PubMed 28508873
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