Skip to main content
Addgene

8047_GFP-Kras2B_ires_puro
(Plasmid #64371)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 64371 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    7432 Bicistronic_GFP_ires_puro
  • Backbone manufacturer
    Theodora Agalioti
  • Backbone size w/o insert (bp) 7432
  • Total vector size (bp) 8047
  • Modifications to backbone
    AgeI, HpaI
  • Vector type
    Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mouse Kras2B
  • Alt name
    Kras
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1356
  • Mutation
    none
  • Entrez Gene
    Kras (a.k.a. K-Ras, K-Ras 2, K-ras, Ki-ras, Kras-2, Kras2, c-K-ras, c-Ki-ras, p21B, ras)
  • Tag / Fusion Protein
    • GFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site SmaI (destroyed during cloning)
  • 5′ sequencing primer ccctcactccttctctagg
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This construct was created in G. Stathopoulos Lab in Patras, Greece by Theodora Agalioti.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    8047_GFP-Kras2B_ires_puro was a gift from Georgios Stathopoulos (Addgene plasmid # 64371 ; http://n2t.net/addgene:64371 ; RRID:Addgene_64371)

  • For your References section:

    Mutant KRAS promotes malignant pleural effusion formation. Agalioti T, Giannou AD, Krontira AC, Kanellakis NI, Kati D, Vreka M, Pepe M, Spella M, Lilis I, Zazara DE, Nikolouli E, Spiropoulou N, Papadakis A, Papadia K, Voulgaridis A, Harokopos V, Stamou P, Meiners S, Eickelberg O, Snyder LA, Antimisiaris SG, Kardamakis D, Psallidas I, Marazioti A, Stathopoulos GT. Nat Commun. 2017 May 16;8:15205. doi: 10.1038/ncomms15205. 10.1038/ncomms15205 PubMed 28508873
Related items:
Commonly requested with: