pIRESpuro3+mRFP-Utr_CH
(Plasmid
#64358)
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Purposebicistronic expression of Utrophin-mRFP and Puro resistance
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 64358 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepIRES Puro3
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameUtrophin-mRFP
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SpeciesH. sapiens (human)
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MutationQ236R; aa1-261 Utrophin present
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Entrez GeneUTRN (a.k.a. DMDL, DRP, DRP1)
- Promoter CMV
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Tags
/ Fusion Proteins
- mRFP (N terminal on insert)
- IRES-Puro (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer pCDH-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pIRESpuro3+mRFP-Utr_CH was a gift from Matthieu Piel (Addgene plasmid # 64358 ; http://n2t.net/addgene:64358 ; RRID:Addgene_64358)
