pIRES Hygro3 LifeAct-mChe
(Plasmid
#64343)
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PurposeBicistronic expression of LifeAct-mCherry fusion and Hygro resistance gene
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 64343 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepIRES Hyg3
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Backbone manufacturerClontech
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameLifeAct
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SpeciesSynthetic
- Promoter CMV
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Tags
/ Fusion Proteins
- mCherry (C terminal on insert)
- IRES-Hygro (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer pCDH-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pIRES Hygro3 LifeAct-mChe was a gift from Matthieu Piel (Addgene plasmid # 64343 ; http://n2t.net/addgene:64343 ; RRID:Addgene_64343)