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7432 Bicistronic_GFP_ires_puro
(Plasmid #64336)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 64336 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    6691 Bicistronic_ires_puro
  • Backbone size w/o insert (bp) 6691
  • Total vector size (bp) 7432
  • Modifications to backbone
    Digest with BglII and XhoI
  • Vector type
    Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP
  • Alt name
    Green Fluorescent Protein
  • Insert Size (bp)
    740
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (destroyed during cloning)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer ccctcactccttctctagg
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This construct was created in G. Stahopoulos lab in Patras, by Theodora Agalioti and represents a retroviral expression vector of Green Fluorescent protein in a bicistronic fashion along with puromycin resistance gene

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    7432 Bicistronic_GFP_ires_puro was a gift from Georgios Stathopoulos (Addgene plasmid # 64336 ; http://n2t.net/addgene:64336 ; RRID:Addgene_64336)

  • For your References section:

    Mutant KRAS promotes malignant pleural effusion formation. Agalioti T, Giannou AD, Krontira AC, Kanellakis NI, Kati D, Vreka M, Pepe M, Spella M, Lilis I, Zazara DE, Nikolouli E, Spiropoulou N, Papadakis A, Papadia K, Voulgaridis A, Harokopos V, Stamou P, Meiners S, Eickelberg O, Snyder LA, Antimisiaris SG, Kardamakis D, Psallidas I, Marazioti A, Stathopoulos GT. Nat Commun. 2017 May 16;8:15205. doi: 10.1038/ncomms15205. 10.1038/ncomms15205 PubMed 28508873
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