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Addgene

pDEST26-USP47
(Plasmid #64326)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 64326 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDEST26
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 7481
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    USP47
  • Alt name
    ubiquitin specific peptidase 47
  • Species
    H. sapiens (human)
  • Entrez Gene
    USP47 (a.k.a. TRFP)
  • Promoter CMV
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST26-USP47 was a gift from Grigory Dianov (Addgene plasmid # 64326 ; http://n2t.net/addgene:64326 ; RRID:Addgene_64326)

  • For your References section:

    USP47 is a deubiquitylating enzyme that regulates base excision repair by controlling steady-state levels of DNA polymerase beta. Parsons JL, Dianova II, Khoronenkova SV, Edelmann MJ, Kessler BM, Dianov GL. Mol Cell. 2011 Mar 4;41(5):609-15. doi: 10.1016/j.molcel.2011.02.016. 10.1016/j.molcel.2011.02.016 PubMed 21362556
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