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Addgene

pTub-Wg
(Plasmid #64321)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 64321 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCaSpeR4
  • Backbone size w/o insert (bp) 12500
  • Total vector size (bp) 12000
  • Modifications to backbone
    alpha-1 tubulin promoter
  • Vector type
    Drosophila Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Wingless
  • Alt name
    Wg
  • Species
    D. melanogaster (fly)
  • Entrez Gene
    wg (a.k.a. Dmel_CG4889, Br, CG4889, D.int-1, DWint-1, DWnt-1, Dint-1, Dm Wg, Dm-1, Dmel\CG4889, Dwnt-1, Gla, I, Sp, WG, WNT, Wg, Wnt, Wnt-1, Wnt1, dWnt, fg, int-1, l(2)02657, l(2)SH1281, l(2)SH2 1281, l(2)rO727, l(2)wg, spd, wgl, wnt, wnt1)
  • Promoter alpha-1 tubulin promoter

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer pCasper-F (5'-gggttttattaacttacat-3')
  • 3′ sequencing primer SV40-splice-R2 (5'-CACAAACAATTAGAATCAGTAG-3')
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTub-Wg was a gift from Roel Nusse (Addgene plasmid # 64321 ; http://n2t.net/addgene:64321 ; RRID:Addgene_64321)

  • For your References section:

    Lipid-independent secretion of a Drosophila Wnt protein. Ching W, Hang HC, Nusse R. J Biol Chem. 2008 Jun 20;283(25):17092-8. doi: 10.1074/jbc.M802059200. Epub 2008 Apr 22. 10.1074/jbc.M802059200 PubMed 18430724
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