pTub-Wg
(Plasmid #64321)

• Purpose: Expresses Drosophila Wingless protein under the tubulin promotor
• Depositing Lab: Roel Nusse
• Publication: Ching et al J Biol Chem. 2008 Jun 20;283(25):17092-8. doi: 10.1074/jbc.M802059200. Epub 2008 Apr 22.

Backbone

• Vector backbone: pCaSpeR4
• Backbone size w/o insert (bp): 12500
• Total vector size (bp): 12000
• Modifications to backbone: alpha-1 tubulin promoter
• Vector type: Drosophila Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Wingless
• Alt name: Wg
• Species: D. melanogaster (fly)
• Entrez Gene: wg (a.k.a. Dmel_CG4889, Br, CG4889, D.int-1, DWint-1, DWnt-1, Dint-1, Dm Wg, Dm-1, Dmel\CG4889, Dwnt-1, Gla, I, Sp, WG, WNT, Wg, Wnt, Wnt-1, Wnt1, dWnt, fg, int-1, l(2)02657, l(2)SH1281, l(2)SH2 1281, l(2)rO727, l(2)wg, spd, wgl, wnt, wnt1)
• Promoter: alpha-1 tubulin promoter

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: pCasper-F (5'-gggttttattaacttacat-3')
• 3′ sequencing primer: SV40-splice-R2 (5'-CACAAACAATTAGAATCAGTAG-3')

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry