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PurposeGFP-tagged mammalian expression of N-terminal domain of Nup153
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 64318 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFP-C3
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4700
- Total vector size (bp) 6648
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNup153
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Alt nameNucleoporin 153kDa
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SpeciesH. sapiens (human)
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MutationN-terminal domain
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Entrez GeneNUP153 (a.k.a. HNUP153, N153)
- Promoter CMV
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Tag
/ Fusion Protein
- GFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer EGFP-C
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFP(C3)-Nup153-N was a gift from Birthe Fahrenkrog (Addgene plasmid # 64318 ; http://n2t.net/addgene:64318 ; RRID:Addgene_64318) -
For your References section:
Structural characterization of altered nucleoporin Nup153 expression in human cells by thin-section electron microscopy. Duheron V, Chatel G, Sauder U, Oliveri V, Fahrenkrog B. Nucleus. 2014;5(6):601-12. doi: 10.4161/19491034.2014.990853. 10.4161/19491034.2014.990853 PubMed 25485891
Map uploaded by the depositor.
