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Addgene

pEGFP(C3)-Nup153-C (880-1475)
(Plasmid #64317)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 64317 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C3
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 6493
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nup153
  • Alt name
    Nucleoporin 153kDa
  • Species
    H. sapiens (human)
  • Mutation
    C-terminal subunit (880-1475)
  • Entrez Gene
    NUP153 (a.k.a. HNUP153, N153)
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XmaI (not destroyed)
  • 5′ sequencing primer EGFP-C
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP(C3)-Nup153-C (880-1475) was a gift from Birthe Fahrenkrog (Addgene plasmid # 64317 ; http://n2t.net/addgene:64317 ; RRID:Addgene_64317)

  • For your References section:

    Structural characterization of altered nucleoporin Nup153 expression in human cells by thin-section electron microscopy. Duheron V, Chatel G, Sauder U, Oliveri V, Fahrenkrog B. Nucleus. 2014;5(6):601-12. doi: 10.4161/19491034.2014.990853. 10.4161/19491034.2014.990853 PubMed 25485891
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