Skip to main content
Addgene

pAc-­His-­zfTpo
(Plasmid #64309)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 64309 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pAcGP67-­B
  • Backbone manufacturer
    BD Biosciences
  • Backbone size w/o insert (bp) 9765
  • Total vector size (bp) 10255
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Thrombopoietin
  • Alt name
    tpo
  • Alt name
    thpo
  • Species
    D. rerio (zebrafish)
  • Mutation
    Thrombopoietin starts at AA37 (AA1-36 encode the signal peptide which is not present in the mature secreted protein)
  • GenBank ID
    EU267076.1
  • Entrez Gene
    thpo (a.k.a. T, tpo)
  • Tag / Fusion Protein
    • (His)6 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (destroyed during cloning)
  • 3′ cloning site HindIII (destroyed during cloning)
  • 5′ sequencing primer AAA TGA TAA CCA TCT CGC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAc-­His-­zfTpo was a gift from Petr Bartunek (Addgene plasmid # 64309 ; http://n2t.net/addgene:64309 ; RRID:Addgene_64309)

  • For your References section:

    Dissection of vertebrate hematopoiesis using zebrafish thrombopoietin. Svoboda O, Stachura DL, Machonova O, Pajer P, Brynda J, Zon LI, Traver D, Bartunek P. Blood. 2014 Jul 10;124(2):220-8. doi: 10.1182/blood-2014-03-564682. Epub 2014 May 28. 10.1182/blood-2014-03-564682 PubMed 24869937
Related items:
Commonly requested with: