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Addgene

pcDNA3.1-Nup88(1-550)
(Plasmid #64282)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 64282 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1 myc-His A
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5493
  • Total vector size (bp) 6046
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nup88
  • Alt name
    nucleoporin 88kDa
  • Species
    H. sapiens (human)
  • Mutation
    N-terminal domain (1-550)
  • Entrez Gene
    NUP88 (a.k.a. FADS4)
  • Promoter CMV
  • Tags / Fusion Proteins
    • myc (C terminal on backbone)
    • his (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGHrev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid contains an F387S mutation in Nup88. The depositor states that this mutation does not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-Nup88(1-550) was a gift from Birthe Fahrenkrog (Addgene plasmid # 64282 ; http://n2t.net/addgene:64282 ; RRID:Addgene_64282)

  • For your References section:

    The nucleoporin Nup88 is interacting with nuclear lamin A. Lussi YC, Hugi I, Laurell E, Kutay U, Fahrenkrog B. Mol Biol Cell. 2011 Apr;22(7):1080-90. doi: 10.1091/mbc.E10-05-0463. Epub 2011 Feb 2. 10.1091/mbc.E10-05-0463 PubMed 21289091
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