pEGFP(C3)-Nup153
(Plasmid #64268)

• Purpose: GFP-tagged mammalian expression of Nup153
• Depositing Lab: Birthe Fahrenkrog
• Publication: Duheron et al Nucleus. 2014;5(6):601-12. doi: 10.4161/19491034.2014.990853.

Backbone

• Vector backbone: pEGFP-C3
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 9127
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NuP153
• Alt name: Nucleoporin 153kDa
• Species: H. sapiens (human)
• Entrez Gene: NUP153 (a.k.a. HNUP153, N153)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XmaI (not destroyed)
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: SV40pA-R

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry