pLVX-Puro-mGFP-WDR34
(Plasmid
#64251)
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PurposeExpresses WDR34 as a fusion with monomeric GFP
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 64251 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLVX-Puro
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Backbone manufacturerClontech
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameWDR34
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Alt nameWD34
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Alt nameFAP133
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SpeciesH. sapiens (human)
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GenBank IDAAH01614.3
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Entrez GeneWDR34 (a.k.a. DIC5, FAP133, SRTD11, bA216B9.3)
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Tag
/ Fusion Protein
- mGFP (N terminal on insert)
Resource Information
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A portion of this plasmid was derived from a plasmid made byOriGene Technologies (Rockville, MD).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Purchased from Origene then amplified by PCR for cloning.
Original OriGene Technologies (Rockville, MD). Number (SC319901)
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLVX-Puro-mGFP-WDR34 was a gift from David Stephens (Addgene plasmid # 64251 ; http://n2t.net/addgene:64251 ; RRID:Addgene_64251) -
For your References section:
Subunit composition of the human cytoplasmic dynein-2 complex. Asante D, Stevenson NL, Stephens DJ. J Cell Sci. 2014 Nov 1;127(21):4774-87. doi: 10.1242/jcs.159038. Epub 2014 Sep 9. 10.1242/jcs.159038 PubMed 25205765