pU6d:sgRNA#5
(Plasmid #64249)

• Purpose: (Empty Backbone) expresses sgRNA under U6d promoter
• Depositing Lab: Wenbiao Chen
• Publication: Yin et al Genetics. 2015 Apr 8. pii: genetics.115.176917.

Backbone

• Vector backbone: pLR-NN
• Backbone manufacturer: Adam Bogdanove, Daniel Voytas lab
• Backbone size (bp): 2568
• Modifications to backbone: zebrafish U6 promoter (U6d) directs a sgRNA expression. BsaI sites were added on both ends of U6d:sgRNA cassettes by PCR and PCR products were inserted into the ApaI/XbaI sites of pLR-NN from the Golden Gate TALEN kit.
• Vector type: CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry