pGGDestTol2LC-2sgRNA
(Plasmid #64240)

• Purpose: (Empty Backbone) destination vector for two U6x:sgRNA cassettes
• Depositing Lab: Wenbiao Chen
• Publication: Yin et al Genetics. 2015 Apr 8. pii: genetics.115.176917.

Backbone

• Vector backbone: pDestTol2LC
• Backbone size (bp): 7083
• Modifications to backbone: One BsaI site in ampicillin sequence is mutated. Two BsaI sites containing adaptors with appropriate overhang (CAGT and TGAG) were sequentially inserted into the NsiI/XhoI and KpnI sites of pDestTol2LC.
• Vector type: CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The destination vector for these U6x:sgRNA cassettes were made first by eliminating the BsaI site in pDestTol2CG2 (Chi-Bin Chien lab), followed by replacing the cmcl2:EGFP (AatII/KpnI) with a cryaa:mCerulean (SacII/KpnI) from phsp70l-loxP-mCherry-STOP-loxP-H2B-GFP_cryaa-cerulean to generate pDestTol2LC (Didier Y. R. Stainier lab)
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • National Institute of Genetics Limited Use Label License for Tol2 System
• Industry Terms:
  • Not Available to Industry