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Cry2 mCherry PGK-1 in pcDNA3.1
(Plasmid #64214)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 64214 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 8896
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Cry2 PHR
  • Alt name
    Cryptochrome 2
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    1494
  • Entrez Gene
    CRY2 (a.k.a. AT1G04400, AT-PHH1, ATCRY2, CRYPTOCHROME 2 APOPROTEIN, F19P19.14, F19P19_14, FHA, PHH1, cryptochrome 2)
  • Promoter CMV
  • Tag / Fusion Protein
    • mcherry (C terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site SmaI (destroyed during cloning)
  • 5′ sequencing primer T7
  • 3′ sequencing primer na
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    PGK1
  • Alt name
    PGKA; MIG10
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1254
  • GenBank ID
    NM_000291.3
  • Entrez Gene
    PGK1 (a.k.a. HEL-S-68p, MIG10, PGKA)

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer na
  • 3′ sequencing primer BGH
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

PGK1 is cloned within the KpnI and XbaI sites

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Cry2 mCherry PGK-1 in pcDNA3.1 was a gift from Narasimhan Gautam (Addgene plasmid # 64214 ; http://n2t.net/addgene:64214 ; RRID:Addgene_64214)

  • For your References section:

    Subcellular optogenetic inhibition of G proteins generates signaling gradients and cell migration. O'Neill PR, Gautam N. Mol Biol Cell. 2014 Aug 1;25(15):2305-14. doi: 10.1091/mbc.E14-04-0870. Epub 2014 Jun 11. 10.1091/mbc.E14-04-0870 PubMed 24920824
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