Skip to main content
Addgene

Cry2 mcherry Gamma 9 in pcDNA3.1
(Plasmid #64208)

Print

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 64208 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 7898
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Cry2
  • Alt name
    Cryptochrome 2
  • Species
    A. thaliana (mustard weed)
  • Entrez Gene
    CRY2 (a.k.a. AT1G04400, AT-PHH1, ATCRY2, CRYPTOCHROME 2 APOPROTEIN, F19P19.14, F19P19_14, FHA, PHH1, cryptochrome 2)
  • Promoter CMV
  • Tag / Fusion Protein
    • mcherry (C terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site SmaI (destroyed during cloning)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    gamma cone
  • Alt name
    GNG9
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    225
  • Entrez Gene
    GNGT2 (a.k.a. G-GAMMA-8, G-GAMMA-C, GNG9, GNGT8, HG3I)
  • Promoter CMV
  • Tag / Fusion Protein
    • n/a

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer BGH reverse
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Cry2 mcherry Gamma 9 in pcDNA3.1 was a gift from Narasimhan Gautam (Addgene plasmid # 64208 ; http://n2t.net/addgene:64208 ; RRID:Addgene_64208)

  • For your References section:

    Subcellular optogenetic inhibition of G proteins generates signaling gradients and cell migration. O'Neill PR, Gautam N. Mol Biol Cell. 2014 Aug 1;25(15):2305-14. doi: 10.1091/mbc.E14-04-0870. Epub 2014 Jun 11. 10.1091/mbc.E14-04-0870 PubMed 24920824
Related items: