CrBlue-mcherry - pcDNA3.1
(Plasmid #64204)

• Purpose: CrBlue fused to mcherry on the C terminus
• Depositing Lab: Narasimhan Gautam
• Publication: Karunarathne et al Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):E1565-74. doi: 10.1073/pnas.1220697110. Epub 2013 Mar 11.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Total vector size (bp): 7082
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CrBlue
• Species: Synthetic
• Insert Size (bp): 971
• Promoter: CMV
• Tag / Fusion Protein:
  • mcherry (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1 (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

CrBlue was cloned into the EcoR1 and NotI sites of pcDNA3.1 and mcherry was fused to the C terminus of CrBlue using the NotI and XbaI sites.