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mcherry Gamma 9 ---pcDNA3.1
(Plasmid #64203)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 64203 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 6358
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    G protein Gamma 9
  • Alt name
    Gng9
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    219
  • GenBank ID
    NM_174328.2
  • Entrez Gene
    GNGT2 (a.k.a. G-GAMMA-8, G-GAMMA-C, GNG9, GNGT8, HG3I)
  • Promoter CMV
  • Tags / Fusion Proteins
    • mcherry (N terminal on insert)
    • 6xHis (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site EcoR1 (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH reverse
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

mCherry was cloned into the HindIII- KpnI sites of pcDNA3.1 and Gamma cone was cloned into the KpnI--EcoR1 sites of pcDNA3.1. Gamma has a His-tag on its N terminus.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mcherry Gamma 9 ---pcDNA3.1 was a gift from Narasimhan Gautam (Addgene plasmid # 64203 ; http://n2t.net/addgene:64203 ; RRID:Addgene_64203)

  • For your References section:

    Optically triggering spatiotemporally confined GPCR activity in a cell and programming neurite initiation and extension. Karunarathne WK, Giri L, Kalyanaraman V, Gautam N. Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):E1565-74. doi: 10.1073/pnas.1220697110. Epub 2013 Mar 11. 10.1073/pnas.1220697110 PubMed 23479634
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