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Addgene

DHFR
(Plasmid #64200)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 64200 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJ411
  • Backbone manufacturer
    DNA 2.0
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Dihydrofolate reductase
  • Alt name
    DHFR
  • Species
    Synthetic; E. coli
  • Tag / Fusion Protein
    • none

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    DHFR was a gift from Vern Schramm (Addgene plasmid # 64200 ; http://n2t.net/addgene:64200 ; RRID:Addgene_64200)

  • For your References section:

    Protein mass-modulated effects in the catalytic mechanism of dihydrofolate reductase: beyond promoting vibrations. Wang Z, Singh P, Czekster CM, Kohen A, Schramm VL. J Am Chem Soc. 2014 Jun 11;136(23):8333-41. doi: 10.1021/ja501936d. Epub 2014 May 27. 10.1021/ja501936d PubMed 24820793
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