pLPC-NMYC-mTRF1deltaBLM
(Plasmid
#64163)
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PurposeRetroviral vector expressing mouse TRF1 lacking BLM-binding motifs with N-terminal Myc tag
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 64163 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLPC-N MYC
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Backbone manufacturerde Lange Lab (Addgene plasmid# 12540)
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Modifications to backbonederived from pLPC-nMYC-mTRF1 delta 313-316
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Vector typeMammalian Expression, Retroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemTRF1
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Alt nametelomeric repeat-binding factor 1
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Alt nameterf1
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SpeciesM. musculus (mouse)
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Insert Size (bp)1200
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MutationDeleted amino acids 313-316 and 339-342 (both BLM-binding motifs)
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GenBank IDNM_009352.2 NP_033378.1
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Entrez GeneTerf1 (a.k.a. Pin2, Trbf1, Trf1)
- Promoter CMV
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Tag
/ Fusion Protein
- Myc (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer pLPC-5 (5'-TAGGCGTGTACGGTGGGA-3')
- 3′ sequencing primer pLPC-3 (5'-CTTGCCAAACCTACAGGT-3') (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that Addgene's sequencing results indicate TRF1 amino acid 312 is present in the plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLPC-NMYC-mTRF1deltaBLM was a gift from Titia de Lange (Addgene plasmid # 64163 ; http://n2t.net/addgene:64163 ; RRID:Addgene_64163) -
For your References section:
TRF1 negotiates TTAGGG repeat-associated replication problems by recruiting the BLM helicase and the TPP1/POT1 repressor of ATR signaling. Zimmermann M, Kibe T, Kabir S, de Lange T. Genes Dev. 2014 Nov 15;28(22):2477-91. doi: 10.1101/gad.251611.114. Epub 2014 Oct 24. 10.1101/gad.251611.114 PubMed 25344324
Map uploaded by the depositor.
![](https://media.addgene.org/data/easy-thumbnails/data/plasmids/64/64163/64163-map_9XOJfJW3g1B3.pdf.940x940_q85_autocrop.png)