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Addgene

pLPC-NMYC-mTRF1deltaBLM
(Plasmid #64163)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 64163 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLPC-N MYC
  • Backbone manufacturer
    de Lange Lab (Addgene plasmid# 12540)
  • Modifications to backbone
    derived from pLPC-nMYC-mTRF1 delta 313-316
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mTRF1
  • Alt name
    telomeric repeat-binding factor 1
  • Alt name
    terf1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1200
  • Mutation
    Deleted amino acids 313-316 and 339-342 (both BLM-binding motifs)
  • GenBank ID
    NM_009352.2 NP_033378.1
  • Entrez Gene
    Terf1 (a.k.a. Pin2, Trbf1, Trf1)
  • Promoter CMV
  • Tag / Fusion Protein
    • Myc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pLPC-5 (5'-TAGGCGTGTACGGTGGGA-3')
  • 3′ sequencing primer pLPC-3 (5'-CTTGCCAAACCTACAGGT-3')
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that Addgene's sequencing results indicate TRF1 amino acid 312 is present in the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLPC-NMYC-mTRF1deltaBLM was a gift from Titia de Lange (Addgene plasmid # 64163 ; http://n2t.net/addgene:64163 ; RRID:Addgene_64163)

  • For your References section:

    TRF1 negotiates TTAGGG repeat-associated replication problems by recruiting the BLM helicase and the TPP1/POT1 repressor of ATR signaling. Zimmermann M, Kibe T, Kabir S, de Lange T. Genes Dev. 2014 Nov 15;28(22):2477-91. doi: 10.1101/gad.251611.114. Epub 2014 Oct 24. 10.1101/gad.251611.114 PubMed 25344324
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