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ptf-Galectin3
(Plasmid #64149)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 64149 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1+mRFP
  • Backbone manufacturer
    Takara-Clontech
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Galectin3
  • Alt name
    LGALS3
  • Species
    H. sapiens (human)
  • Entrez Gene
    LGALS3 (a.k.a. CBP35, GAL3, GALBP, GALIG, L31, LGALS2, MAC2)
  • Promoter CMV
  • Tags / Fusion Proteins
    • mRFP (N terminal on insert)
    • EGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (unknown if destroyed)
  • 3′ cloning site SalI (unknown if destroyed)
  • 5′ sequencing primer N/A
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ptf-Galectin3 was a gift from Tamotsu Yoshimori (Addgene plasmid # 64149 ; http://n2t.net/addgene:64149 ; RRID:Addgene_64149)

  • For your References section:

    Autophagy sequesters damaged lysosomes to control lysosomal biogenesis and kidney injury. Maejima I, Takahashi A, Omori H, Kimura T, Takabatake Y, Saitoh T, Yamamoto A, Hamasaki M, Noda T, Isaka Y, Yoshimori T. EMBO J. 2013 Aug 28;32(17):2336-47. doi: 10.1038/emboj.2013.171. Epub 2013 Aug 6. 10.1038/emboj.2013.171 PubMed 23921551
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