pCold-TF-hPLEKHM1
(Plasmid #64146)

• Purpose: For protein expression in E.coli
• Depositing Lab: Tamotsu Yoshimori
• Publication: Tabata et al Mol Biol Cell. 2010 Dec;21(23):4162-72. doi: 10.1091/mbc.E10-06-0495. Epub 2010 Oct 13.

Backbone

• Vector backbone: pCold TF
• Backbone manufacturer: Takara-Clontech
• Backbone size w/o insert (bp): 5769
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PLEKHM1
• Alt name: Pleckstrin homology domain-containing family M member 1
• Species: H. sapiens (human)
• Entrez Gene: PLEKHM1 (a.k.a. AP162, B2, OPTA3, OPTB6)
• Promoter: cspA
• Tags / Fusion Proteins:
  • 6xHis-tag (N terminal on insert)
  • Trigger Factor (TF) (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (unknown if destroyed)
• 3′ cloning site: XbaI (unknown if destroyed)
• 5′ sequencing primer: N/A

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Kazusa DNA Research Institute (Kisarazu, Japan)
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry