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Addgene

pBAD_His B_QuasAr1 Notes

Addgene diagnostic digest

This gel depicts the NEB 1kb DNA ladder and a digestion of plasmid 64135 with BamHI-HF and NdeI.

We see the expected banding patterns:

BamHI-HF single digest: 1 band at 4.7

NdeI single digest: 1 band at 4.7

BamHI-HF / NdeI double digest: 2 bands - 3.2 and 1.5 KB