NLS-dCas9-trCIB1
(Plasmid #64119)

• Purpose: Photoactivatable transcription system. Expression of genomic anchor probe, containing dCas9 and CIB1
• Depositing Lab: Moritoshi Sato
• Publication: Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi: 10.1016/j.chembiol.2014.12.011. Epub 2015 Jan 22.

Backbone

• Vector backbone: pcDNA3.1/V5-His A
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: dCas9-trCIB1 fusion
• Species: Synthetic
• Insert Size (bp): 5277
• Mutation: dCas9 (D10A H840A, catalytically inactive), trCIB1 (C terminally truncated CIB1, delta 308-334)
• Promoter: CMV
• Tags / Fusion Proteins:
  • HA (N terminal on insert)
  • NLS

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH Reverse

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry