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Addgene

pHAGE-TO-nmdCas9-3XmCherry Notes

Addgene Diagnostic Digest

This gel depicts the NEB 1kb extend ladder and a digestion of the plasmid pHAGE-TO-nmdCas9-3XmCherry with the following enzymes: EcoRI-HF and NotI-HF.

With EcoRI-HF, we expect one band at 12.1 kb.
With EcoRI-HF/NotI-HF, we expect two bands at 8 and 4 kb.

We see the expected pattern