pAAV-synP-FLEX-TVA66T-EGFP
(Plasmid #64096)

• Purpose: Expresses TVA66T-EGFP under the synapsin promotor
• Depositing Lab: Ian Wickersham
• Publication: Genetic Neuroengineering Group Plasmids (unpublished)

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Backbone

• Vector backbone: pAAV
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 4440
• Total vector size (bp): 5595
• Vector type: Mammalian Expression, AAV, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TVA66T-EGFP
• Alt name: TVA950 E66T-EGFP
• Species: Synthetic
• Insert Size (bp): 1155
• Mutation: Glutamic acid 66 to Threonine
• Promoter: synapsin
• Tag / Fusion Protein:
  • EGFP (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: AGGCGCGAGATAGGg
• 3′ sequencing primer: cagcgtatccacatagcg

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry