pAAV-synP-FLEX-TVA66T-EGFP
(Plasmid
#64096)
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PurposeExpresses TVA66T-EGFP under the synapsin promotor
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 64096 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 4440
- Total vector size (bp) 5595
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Vector typeMammalian Expression, AAV, Cre/Lox
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTVA66T-EGFP
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Alt nameTVA950 E66T-EGFP
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SpeciesSynthetic
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Insert Size (bp)1155
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MutationGlutamic acid 66 to Threonine
- Promoter synapsin
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Tag
/ Fusion Protein
- EGFP (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer AGGCGCGAGATAGGg
- 3′ sequencing primer cagcgtatccacatagcg (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-synP-FLEX-TVA66T-EGFP was a gift from Ian Wickersham (Addgene plasmid # 64096 ; http://n2t.net/addgene:64096 ; RRID:Addgene_64096)