VZV ORF 37(gH)
(Plasmid
#64081)
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Purposeeukaryotic expression plasmid for VZV glycoprotein H
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 64081 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCI-neo
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 5472
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameVZV glycoprotein H
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Alt nameVZV ORF 37
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Specieshuman herpesvirus type 3 (varicella zoster virus)
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Insert Size (bp)2523
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GenBank ID
- Promoter CMV IE with intron
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer EBV-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
works well to express the virus glycoprotein
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
VZV ORF 37(gH) was a gift from Randall Cohrs (Addgene plasmid # 64081 ; http://n2t.net/addgene:64081 ; RRID:Addgene_64081) -
For your References section:
Human anti-varicella-zoster virus (VZV) recombinant monoclonal antibody produced after Zostavax immunization recognizes the gH/gL complex and neutralizes VZV infection. Birlea M, Owens GP, Eshleman EM, Ritchie A, Traktinskiy I, Bos N, Seitz S, Azarkh Y, Mahalingam R, Gilden D, Cohrs RJ. J Virol. 2013 Jan;87(1):415-21. doi: 10.1128/JVI.02561-12. Epub 2012 Oct 17. 10.1128/JVI.02561-12 PubMed 23077312