human MTAP
(Plasmid
#64077)
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PurposeExpression of human 5'-methylthioadenosine phosphorylase
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 64077 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepJ414
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Backbone manufacturerDNA 2.0
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert name5'-methylthioadenosine phosphorylase
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Alt nameMTAP
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SpeciesH. sapiens (human), Synthetic
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Entrez GeneMTAP (a.k.a. BDMF, DMSFH, DMSMFH, HEL-249, LGMBF, MSAP, c86fus)
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Tags
/ Fusion Proteins
- Histidine tag (N terminal on insert)
- TEV (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
human MTAP was a gift from Vern Schramm (Addgene plasmid # 64077 ; http://n2t.net/addgene:64077 ; RRID:Addgene_64077) -
For your References section:
Entropy-driven binding of picomolar transition state analogue inhibitors to human 5'-methylthioadenosine phosphorylase. Guan R, Ho MC, Brenowitz M, Tyler PC, Evans GB, Almo SC, Schramm VL. Biochemistry. 2011 Nov 29;50(47):10408-17. doi: 10.1021/bi201321x. Epub 2011 Nov 7. 10.1021/bi201321x PubMed 21985704