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Addgene

human MTAP
(Plasmid #64077)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 64077 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pJ414
  • Backbone manufacturer
    DNA 2.0
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    5'-methylthioadenosine phosphorylase
  • Alt name
    MTAP
  • Species
    H. sapiens (human), Synthetic
  • Entrez Gene
    MTAP (a.k.a. BDMF, DMSFH, DMSMFH, HEL-249, LGMBF, MSAP, c86fus)
  • Tags / Fusion Proteins
    • Histidine tag (N terminal on insert)
    • TEV (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    human MTAP was a gift from Vern Schramm (Addgene plasmid # 64077 ; http://n2t.net/addgene:64077 ; RRID:Addgene_64077)
  • For your References section:

    Entropy-driven binding of picomolar transition state analogue inhibitors to human 5'-methylthioadenosine phosphorylase. Guan R, Ho MC, Brenowitz M, Tyler PC, Evans GB, Almo SC, Schramm VL. Biochemistry. 2011 Nov 29;50(47):10408-17. doi: 10.1021/bi201321x. Epub 2011 Nov 7. 10.1021/bi201321x PubMed 21985704