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Human PNP his-tagged
(Plasmid #64076)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 64076 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    T7/NT Topo
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Purine Nucleoside Phosphorylase
  • Alt name
    PNP
  • Species
    H. sapiens (human)
  • GenBank ID
    AH001522.1
  • Entrez Gene
    PNP (a.k.a. NP, PRO1837, PUNP)
  • Tag / Fusion Protein
    • Histidine tag (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Human PNP his-tagged was a gift from Vern Schramm (Addgene plasmid # 64076 ; http://n2t.net/addgene:64076 ; RRID:Addgene_64076)

  • For your References section:

    Achieving the ultimate physiological goal in transition state analogue inhibitors for purine nucleoside phosphorylase. Lewandowicz A, Tyler PC, Evans GB, Furneaux RH, Schramm VL. J Biol Chem. 2003 Aug 22;278(34):31465-8. Epub 2003 Jul 3. 10.1074/jbc.C300259200 PubMed 12842889
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