pDyn1
(Plasmid #64067)

• Purpose: Human cytoplasmic dynein heavy chain (DYNC1H1) codon optimized for Sf9 expression with a SNAPf tag for fluorescent labeling and a TEV-cleavable His-ZZ tag for purification (His-ZZ-TCS-SNAPf-DYNC1H1).
• Depositing Lab: Andrew Carter
• Publication: Schlager et al EMBO J. 2014 Sep 1;33(17):1855-68. doi: 10.15252/embj.201488792. Epub 2014 Jul 1.

Backbone

• Vector backbone: pACEBac1
• Backbone manufacturer: EMBL
• Backbone size w/o insert (bp): 3938
• Total vector size (bp): 17876
• Vector type: Insect Expression, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Gentamicin, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: DYNC1H1
• Alt name: Cytoplasmic dynein heavy chain 1
• Species: H. sapiens (human)
• Insert Size (bp): 13938
• Mutation: Codon optimized for Sf9
• GenBank ID: NM_001376.4
• Entrez Gene: DYNC1H1 (a.k.a. CDCBM13, CMT2O, DHC1, DHC1a, DNCH1, DNCL, DNECL, DYHC, Dnchc1, HL-3, SMALED1, p22)
• Tag / Fusion Protein:
  • His-ZZ-TCS-SNAPf (N terminal on backbone)

Resource Information

• Supplemental Documents:
  • pDyn1 GenBank
• Addgene Notes:
  • Addgene Diagnostic Test
• A portion of this plasmid was derived from a plasmid made by: Synthesized commercially by Epoch Life Science.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry