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EcM2.1 dtolC bla::zeoR
(Bacterial strain #64054)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Bacterial Strain 64054 Bacteria in agar stab 1 $85
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Backbone

  • Vector backbone
    N/A

Growth in Bacteria

  • Bacterial Resistance(s)
    Bleocin (Zeocin), 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    This is a strain
  • Growth instructions
    zeocinR, sdsS, requires biotin supplementation in minimal media, temperature sensitive grow below 34 degrees C.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    see comments

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Escherichia coli MG1655 mutS_fs dnaG_Q576A exoX- xonA- xseA- 1255700::tolQRA Δ(ybhB-bioAB)::[λcI857 N(cro-ea59)::tetR-(bla::zeoR)] tolC-

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EcM2.1 dtolC bla::zeoR was a gift from George Church (Addgene plasmid # 64054)

  • For your References section:

    Rational optimization of tolC as a powerful dual selectable marker for genome engineering. Gregg CJ, Lajoie MJ, Napolitano MG, Mosberg JA, Goodman DB, Aach J, Isaacs FJ, Church GM. Nucleic Acids Res. 2014 Apr;42(7):4779-90. doi: 10.1093/nar/gkt1374. Epub 2014 Jan 22. 10.1093/nar/gkt1374 PubMed 24452804
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