pCfB2793
(Plasmid #64043)

• Purpose: pCfB2793 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.
• Depositing Labs: Irina Borodina, Jerome Maury
• Publication: Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi: 10.1371/journal.pone.0150394. eCollection 2016.

Backbone

• Vector backbone: see publication
• Total vector size (bp): 6608
• Vector type: Yeast Expression, Cre/Lox
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: GFP
• Promoter: TEF1 promoter

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: TEF-seq

Resource Information

• Supplemental Documents:
  • Annotated Genbank file p2793

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please see previous FEMS Yeast Research paper for more details:
http://femsyr.oxfordjournals.org/content/14/2/238

Feature list:
T CYC1 Terminator 6..195
T K.l. URA3 Terminator 303..425
Degradation tag 426..476
K. lactis URA3 CDS without STOP codon 477..1277
K. lactis URA3 promoter 1278..1776
Consensus Ty2 3 1966..2152
pUC ori 2939..3582
Consensus Ty2 5 4987..5215
T ADH1 Terminator 5242..5436
pTEF1 Promoter 5455..5874
GFP CDS 5884..6600