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Addgene

pcDNA3-EngPDm
(Plasmid #64040)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 64040 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5446
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pax8 paired domain
  • Alt name
    paired box 8
  • Species
    M. musculus (mouse)
  • Mutation
    paired domain, aa 1-147, with aa 30 to 32 changed to Ala (creating a NotI restriction site)
  • Entrez Gene
    Pax8 (a.k.a. Pax-8)
  • Promoter CMV
  • Tag / Fusion Protein
    • Eng (N-terminal repressor domain of the drosophila engrailed protein) (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH Reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There is a BamHI site in between the Eng and PDm coding sequences.

Drosophila Engrailed (Eng)contains H22P and L158P compared to closest reference sequence (AAA65478.1). Depositor states that these mutation should not affect function.

Mouse Pax8 contains a P2A mutation. Depositor states that this mutation should not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-EngPDm was a gift from Daniel Christophe (Addgene plasmid # 64040 ; http://n2t.net/addgene:64040 ; RRID:Addgene_64040)

  • For your References section:

    Human Thyroid Oxidases genes promoter activity in thyrocytes does not appear to be functionally dependent on Thyroid Transcription Factor-1 or Pax8. Christophe-Hobertus C, Christophe D. Mol Cell Endocrinol. 2007 Jan 29;264(1-2):157-63. Epub 2006 Dec 19. 10.1016/j.mce.2006.11.005 PubMed 17182173
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