hSyn-FAS-DO-ReaChR-mCitrine-WPRE
(Plasmid
#64035)
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PurposeExpresses ReaChR under hSyn promoter. FAS-DO turns off expression in Cre-positive cells. FAS is compatible with other traditional Flex/DIO constructs
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 64035 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV
- Backbone size w/o insert (bp) 2750
- Total vector size (bp) 6450
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberUnknown
Gene/Insert
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Gene/Insert namehSyn-FAS-DO-ReaChR-mCitrine-WPRE
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Alt nameFAS-DO-ReaChR
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SpeciesSynthetic
- Promoter hSyn
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Tag
/ Fusion Protein
- ReaChR tagged with mCitrine (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer unknown
- 3′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Made by Kim Ritola at Janelia Farm Research Campus, HHMI, for Bryan (Mac) Hooks in Karel Svoboda's lab.
FAS-DO site is for compatibility with FLEX/DIO constructs with traditional loxP sites (Saunders, A., Johnson, C. and Sabatini, B.L. 2012. Novel recombinant adeno-associated viruses for Cre activated and inactivated transgene expression. Front. in Neural Circuits 6:47)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
hSyn-FAS-DO-ReaChR-mCitrine-WPRE was a gift from Karel Svoboda (Addgene plasmid # 64035 ; http://n2t.net/addgene:64035 ; RRID:Addgene_64035)
Map uploaded by the depositor.
![](https://media.addgene.org/data/easy-thumbnails/data/plasmids/64/64035/64035-map_5Kquq9ejqOHa.pdf.940x940_q85_autocrop.png)