hSyn-FAS-DO-ReaChR-mCitrine-WPRE
(Plasmid #64035)

• Purpose: Expresses ReaChR under hSyn promoter. FAS-DO turns off expression in Cre-positive cells. FAS is compatible with other traditional Flex/DIO constructs
• Depositing Lab: Karel Svoboda
• Publication: hSyn-FAS-DO-ReaChR-mCitrine-WPRE (unpublished)

Backbone

• Vector backbone: pAAV
• Backbone size w/o insert (bp): 2750
• Total vector size (bp): 6450
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: hSyn-FAS-DO-ReaChR-mCitrine-WPRE
• Alt name: FAS-DO-ReaChR
• Species: Synthetic
• Promoter: hSyn
• Tag / Fusion Protein:
  • ReaChR tagged with mCitrine (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: unknown
• 3′ sequencing primer: unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Made by Kim Ritola at Janelia Farm Research Campus, HHMI, for Bryan (Mac) Hooks in Karel Svoboda's lab.
FAS-DO site is for compatibility with FLEX/DIO constructs with traditional loxP sites (Saunders, A., Johnson, C. and Sabatini, B.L. 2012. Novel recombinant adeno-associated viruses for Cre activated and inactivated transgene expression. Front. in Neural Circuits 6:47)