APRT
(Plasmid
#64028)
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PurposeExpression of S. crervisiae strain APRTase in E. coli. Contains N-terminal HistidineX6 tag.
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 64028 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepQE32
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Backbone manufacturerQiagen
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameadenine phosphoribosyltransferase
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Alt nameAPRTase
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Alt nameAPRT
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SpeciesS. cerevisiae (budding yeast)
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Entrez GeneAPT1 (a.k.a. YML022W)
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Tag
/ Fusion Protein
- Histidine x 6 (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
APRT was a gift from Vern Schramm (Addgene plasmid # 64028 ; http://n2t.net/addgene:64028 ; RRID:Addgene_64028) -
For your References section:
Structural analysis of adenine phosphoribosyltransferase from Saccharomyces cerevisiae. Shi W, Tanaka KS, Crother TR, Taylor MW, Almo SC, Schramm VL. Biochemistry. 2001 Sep 11;40(36):10800-9. bi010465h [pii] PubMed 11535055