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Purpose(Empty Backbone) Plasmid for integration into neutral site 2 of Synechocystis 6803
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 64026 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepJ344
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Backbone manufacturerDNA 2.0
- Backbone size (bp) 3792
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Vector typeBacterial Expression, Synthetic Biology
- Promoter PnrsB
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEERM4 Cm was a gift from Pia Lindberg (Addgene plasmid # 64026 ; http://n2t.net/addgene:64026 ; RRID:Addgene_64026) -
For your References section:
Metabolic Engineering of Synechocystis sp. PCC 6803 for Production of the Plant Diterpenoid Manoyl Oxide. Englund E, Andersen-Ranberg J, Miao R, Hamberger B, Lindberg P. ACS Synth Biol. 2015 Jul 13. 10.1021/acssynbio.5b00070 PubMed 26133196