punc503GFP
(Plasmid #64021)

• Purpose: 503-bp promoter of zebrafish unc-45b drives muscle-specific GFP expression
• Depositing Labs: Joachim Berger, Peter Currie
• Publication: Berger et al Genesis. 2013 Jun;51(6):443-7. doi: 10.1002/dvg.22385. Epub 2013 Mar 26.

Backbone

• Vector backbone: pDestTol2pA2
• Backbone manufacturer: Kwan et al., 2007
• Vector type: Gateway

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: unc-45b: GFP
• Species: D. rerio (zebrafish)
• Insert Size (bp): 1533
• Entrez Gene: unc45b (a.k.a. Unc-45B, cb393, fb37f02, unc45r, wu:fa05a03, wu:fa10f03, wu:fb37f02, wu:fb96f06, wu:fc10f04)
• Promoter: 503-bp promoter of zebrafish unc-45b

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13forward

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • National Institute of Genetics Limited Use Label License for Tol2 System
• Industry Terms:
  • Not Available to Industry