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Purpose5' Gateway entry vector with muscle-specific promoter of zebrafish unc-45b
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 64020 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDONRP4-P1R
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Backbone manufacturerInvitrogen
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Vector typegateway
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemuscle-specific promoter of zebrafish unc-45b
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)503
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Entrez Geneunc45b (a.k.a. Unc-45B, cb393, fb37f02, unc45r, wu:fa05a03, wu:fa10f03, wu:fb37f02, wu:fb96f06, wu:fc10f04)
- Promoter 503-bp promoter of zebrafish unc-45b
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13Forward
- 3′ sequencing primer M13reverse (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p5E_unc503 was a gift from Joachim Berger & Peter Currie (Addgene plasmid # 64020 ; http://n2t.net/addgene:64020 ; RRID:Addgene_64020) -
For your References section:
503unc, a small and muscle-specific zebrafish promoter. Berger J, Currie PD. Genesis. 2013 Jun;51(6):443-7. doi: 10.1002/dvg.22385. Epub 2013 Mar 26. 10.1002/dvg.22385 PubMed 23444339
