pST50Trc4-CBPHISNDHFR
(Plasmid
#63999)
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Purpose(Empty Backbone) Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 63999 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepST50Trc
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Vector typeBacterial Expression
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Tag
/ Fusion Protein
- 6xHis & Calmodulin Binding Peptide (CBP); TEV cleavable (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- 6xHis & Calmodulin Binding Peptide (CBP); TEV cleavable (N terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Replace DHFR with GOI using BamHI/NgoMIV; Clone out cassette using BspEI/MluI
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pST50Trc4-CBPHISNDHFR was a gift from Song Tan (Addgene plasmid # 63999 ; http://n2t.net/addgene:63999 ; RRID:Addgene_63999) -
For your References section:
The pST44 polycistronic expression system for producing protein complexes in Escherichia coli. Tan S, Kern RC, Selleck W. Protein Expr Purif. 2005 Apr;40(2):385-95. 10.1016/j.pep.2004.12.002 PubMed 15766881
