pYC62
(Plasmid
#63927)
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Purpose(Empty Backbone) Recipient replicative vector to construct HA tagged fusions
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 63927 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMJ22
- Backbone size (bp) 4509
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Modifications to backboneDigested and clonning of HA casete as BamHI/BglII fragment
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Vector typeYeast Expression
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Selectable markersNourseothricin
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Tag
/ Fusion Protein
- HA
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH10B
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYC62 was a gift from Irene Castano Navarro (Addgene plasmid # 63927 ; http://n2t.net/addgene:63927 ; RRID:Addgene_63927) -
For your References section:
Expression vectors for C-terminal fusions with fluorescent proteins and epitope tags in Candida glabrata. Yanez-Carrillo P, Orta-Zavalza E, Gutierrez-Escobedo G, Patron-Soberano A, De Las Penas A, Castano I. Fungal Genet Biol. 2015 May 15. pii: S1087-1845(15)00087-0. doi: 10.1016/j.fgb.2015.04.020. 10.1016/j.fgb.2015.04.020 PubMed 25986172