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Addgene

pCSCX
(Plasmid #63918)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 63918 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR2.1
  • Backbone manufacturer
    Life Technologies
  • Backbone size w/o insert (bp) 4059
  • Total vector size (bp) 7988
  • Vector type
    Bacterial cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cscAKB
  • Species
    Escherichia coli W
  • Insert Size (bp)
    3929
  • GenBank ID
    CP002185

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The transferrable sucrose utilization cassette contains cscA (invertase), cscK (d-fructokinase), and cscB (sucrose permease).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCSCX was a gift from Claudia Vickers (Addgene plasmid # 63918 ; http://n2t.net/addgene:63918 ; RRID:Addgene_63918)

  • For your References section:

    A transferable sucrose utilization approach for non-sucrose-utilizing Escherichia coli strains. Bruschi M, Boyes SJ, Sugiarto H, Nielsen LK, Vickers CE. Biotechnol Adv. 2012 Sep-Oct;30(5):1001-10. doi: 10.1016/j.biotechadv.2011.08.019. Epub 2011 Sep 1. 10.1016/j.biotechadv.2011.08.019 PubMed 21907272
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