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Addgene

pYC25
(Plasmid #63914)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 63914 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYC12
  • Backbone size (bp) 5693
  • Modifications to backbone
    Digested and clonning of YFP casete as BamHI/BglII fragment
  • Vector type
    Yeast Expression
  • Promoter TEF1 (Saccharomyces cerevisiae)
  • Selectable markers
    URA3
  • Tag / Fusion Protein
    • YFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYC25 was a gift from Irene Castano Navarro (Addgene plasmid # 63914 ; http://n2t.net/addgene:63914 ; RRID:Addgene_63914)

  • For your References section:

    Expression vectors for C-terminal fusions with fluorescent proteins and epitope tags in Candida glabrata. Yanez-Carrillo P, Orta-Zavalza E, Gutierrez-Escobedo G, Patron-Soberano A, De Las Penas A, Castano I. Fungal Genet Biol. 2015 May 15. pii: S1087-1845(15)00087-0. doi: 10.1016/j.fgb.2015.04.020. 10.1016/j.fgb.2015.04.020 PubMed 25986172
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